Human Gene Set: CASTELLANO_HRAS_AND_NRAS_TARGETS_UP

For the Mouse gene set with the same name, see CASTELLANO_HRAS_AND_NRAS_TARGETS_UP

Standard name CASTELLANO_HRAS_AND_NRAS_TARGETS_UP
Systematic name M18773
Brief description Genes up-regulated in MEF cells (embryonic fibroblasts) isolated from HRAS and NRAS [GeneID=3265;4893] double knockout mice.
Full description or abstract We characterized differential gene expression profiles of fibroblast cell lines harboring single or double-homozygous null mutations in H-ras and N-ras. Whereas the expression level of the individual H-, N- and K-ras genes appeared unaffected by the presence or absence of the other ras loci, significant differences were observed between the expression profiles of cells missing N-ras and/or H-ras. Absence of N-ras produced much stronger effects than absence of H-ras over the profile of the cellular transcriptome. N-ras(-/-) and H-ras(-/-) fibroblasts displayed rather antagonistic expression profiles and the transcriptome of H-ras(-/-) cells was significantly closer to that of wild-type fibroblasts than to that of N-ras(-/-) cells. Classifying all differentially expressed genes into functional categories suggested specific roles for H-Ras and N-Ras. It was particularly striking in N-ras(-/-) cells the upregulation of a remarkable number of immunity-related genes, as well as of several loci involved in apoptosis. Reverse-phase protein array assays demonstrated in the same N-ras(-/-) cells the overexpression and nuclear migration of tyrosine phosphorylated signal transducer and activator of transcription 1 (Stat1) which was concomitant with transcriptional activation mediated by interferon-stimulated response elements. Significantly enhanced numbers of apoptotic cells were also detected in cultures of N-ras(-/-) cells. Our data support the notion that different Ras isoforms play functionally distinct cellular roles and indicate that N-Ras is significantly involved in immune modulation/host defense and apoptotic responses.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 16909116   Authors: Castellano E,De Las Rivas J,Guerrero C,Santos E
Exact source Table 3: delta(i) > 0
Related gene sets (show 5 additional gene sets from the source publication)

(show 1 gene sets from the same authors)
External links
Filtered by similarity ?
Source species Mus musculus
Contributed by Leona Saunders (MSigDB Team)
Source platform or
identifier namespace
AFFY_MG_U74
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)

Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 6 genes
Gene families ? Categorize these 6 genes by gene family
Show members (show 6 source identifiers mapped to 6 genes)
Version history  

See MSigDB license terms here. Please note that certain gene sets have special access terms.