Gene Set: CHIARADONNA_NEOPLASTIC_TRANSFORMATION_KRAS_CDC25_DN

Standard name CHIARADONNA_NEOPLASTIC_TRANSFORMATION_KRAS_CDC25_DN
Systematic name M5312
Brief description Genes down-regulated in NIH3T3 cells (fibroblasts) transformed by activated KRAS [GeneID=3845] vs those reverted to normal cells upon over-expression of a dominant negative form of CDC25 [GeneID=5923].
Full description or abstract Mutational activation of ras genes is required for the onset and maintenance of different malignancies. Here we show, using a combination of molecular physiology, nutritional perturbations and transcriptional profiling, that full penetrance of phenotypes related to oncogenic Ras activation, including the shift of carbon metabolism towards fermentation and upregulation of key cell cycle regulators, is dependent upon glucose availability. These responses are induced by Ras activation, being specifically reverted by downregulation of the Ras pathway obtained through the expression of a dominant-negative Ras-specific guanine nucleotide exchange protein. Our data allow to link directly to ras activation the alteration in energy metabolism of cancer cells, their fragility towards glucose shortage and ensuing apoptotic death.
Collection C2: curated gene sets
      CGP: chemical and genetic perturbations
Source publication Pubmed 16607279   Authors: Chiaradonna F,Sacco E,Manzoni R,Giorgio M,Vanoni M,Alberghina L
Exact source Table 1S: kras-DN Down regulated genes
Related gene sets (show 5 additional gene sets from the source publication)
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Organism Mus musculus
Contributed by Lauren Kazmierski (Broad Institute)
Source platform MG_U74Av2
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Compendia expression profiles Human tissue compendium (Novartis)
NCI-60 cell lines (National Cancer Institute)
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