Human Gene Set: GREENBAUM_E2A_TARGETS_UP

For the Mouse gene set with the same name, see GREENBAUM_E2A_TARGETS_UP

Standard name GREENBAUM_E2A_TARGETS_UP
Systematic name M1490
Brief description Genes up-regulated in pre-B lymphocytes upon Cre-Lox knockout of E2A [GeneID=6929].
Full description or abstract The transcription factors encoded by the E2A gene have been shown to play essential roles in the initiation and progression of lymphocyte development. However, there is still a lack of comprehensive understanding of E2A downstream genes in B-cell development. We previously developed a gene tagging-based chromatin immunoprecipitation (ChIP) system to directly evaluate E2A target genes in B-cell development. Here, we have improved this ChIP strategy and used it in conjunction with microarray analysis on E2A-deficient pre-B-cell lines to determine E2A target genes in lymphocyte development. Both microarray data and ChIP studies confirmed that E2A directly controls IgH gene expression. The microarray assay also revealed genes that were significantly up-regulated after E2A disruption. ChIP analysis showed that E2A was most likely to be directly involved in repression of some of these target genes such as Nfil3 and FGFR2. An inducible E2A reconstitution system further demonstrated that E2A-mediated repression of Nfil3 and FGFR2 was reversible. Collectively, these findings indicate that E2A is a positive regulator for one set of genes and a negative regulator for another set of genes in developing B lymphocytes.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 15310760   Authors: Greenbaum S,Lazorchak AS,Zhuang Y
Exact source Table 1
Related gene sets (show 1 additional gene sets from the source publication)

(show 4 gene sets from the same authors)
External links
Filtered by similarity ?
Source species Mus musculus
Contributed by Kevin Vogelsang (MSigDB Team)
Source platform or
identifier namespace
MOUSE_SEQ_ACCESSION
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)

Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 33 genes
Gene families ? Categorize these 33 genes by gene family
Show members (show 35 source identifiers mapped to 33 genes)
Version history 3.1: First introduced

See MSigDB license terms here. Please note that certain gene sets have special access terms.