Gene Set: HATADA_METHYLATED_IN_LUNG_CANCER_UP

Standard name HATADA_METHYLATED_IN_LUNG_CANCER_UP
Systematic name M19508
Brief description Genes with hypermethylated DNA in lung cancer samples.
Full description or abstract DNA methylation in the promoter region of a gene is associated with a loss of that gene's expression and plays an important role in gene silencing. The inactivation of tumor-suppressor genes by aberrant methylation in the promoter region is well recognized in carcinogenesis. However, there has been little study in this area when it comes to genome-wide profiling of the promoter methylation. Here, we developed a genome-wide profiling method called Microarray-based Integrated Analysis of Methylation by Isoschizomers to analyse the DNA methylation of promoter regions of 8091 human genes. With this method, resistance to both the methylation-sensitive restriction enzyme HpaII and the methylation-insensitive isoschizomer MspI was compared between samples by using a microarray with promoter regions of the 8091 genes. The reliability of the difference in HpaII resistance was judged using the difference in MspI resistance. We demonstrated the utility of this method by finding epigenetic mutations in cancer. Aberrant hypermethylation is known to inactivate tumour suppressor genes. Using this method, we found that frequency of the aberrant promoter hypermethylation in cancer is higher than previously hypothesized. Aberrant hypomethylation is known to induce activation of oncogenes in cancer. Genome-wide analysis of hypomethylated promoter sequences in cancer demonstrated low CG/GC ratio of these sequences, suggesting that CpG-poor genes are sensitive to demethylation activity in cancer.
Collection C2: curated gene sets
      CGP: chemical and genetic perturbations
Source publication Pubmed 16407832   Authors: Hatada I,Fukasawa M,Kimura M,Morita S,Yamada K,Yoshikawa T,Yamanaka S,Endo C,Sakurada A,Sato M,Kondo T,Horii A,Ushijima T,Sasaki H
Exact source Table 2S: hypermethylated
Related gene sets (show 1 additional gene sets from the source publication)

(show 15 gene sets from the same authors)
External links  
Organism Homo sapiens
Contributed by Arthur Liberzon (Broad Institute)
Source platform SEQ_ACCESSION
Dataset references  
Download gene set format: grp | text | gmt | gmx | xml
Compute overlaps (show collections to investigate for overlap with this gene set)
Compendia expression profiles Human tissue compendium (Novartis)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 390 genes
Gene families Categorize these 390 genes by gene family
Show members (show 400 members mapped to 390 genes)
Version history 3.0: First introduced

See MSigDB license terms here. Please note that certain gene sets have special access terms.