Gene Set: JAZAG_TGFB1_SIGNALING_UP

Standard name JAZAG_TGFB1_SIGNALING_UP
Systematic name M7160
Brief description Genes up-regulated in PANC-1-puro cells (pancreatic cancer) stimulated by TGF1B [GeneID=7040] for 2 h.
Full description or abstract The transforming growth factor-beta (TGF-beta)-Smad signaling pathway inhibits the growth of human epithelial cells and plays a role in tumor suppression. The Smad4 gene is mutated or deleted in 50% of pancreatic cancers. In this study, we succeeded in establishing Smad4 knockdown (S4KD) pancreatic cancer cell lines using the stable RNA interference (RNAi) method. Smad4 protein expression was reduced dramatically and TGF-beta-Smad signaling was markedly inhibited in the S4KD cell lines. The S4KD and control cells were stimulated with TGF-beta and analysed using a cDNA microarray that contained 3756 genes, in order to screen for target molecules downstream of TGF-beta. The microarray analysis revealed that 187 S4KD genes and 155 genes in the control cells were regulated immediately upon TGF-beta stimulation. Quantitative RT-PCR analysis on several of these genes produced results that corroborated the outcome of the microarray analysis. Most of the genes in the S4KD and control cells identified by the array differed, which suggests signaling pathways that differ according to Smad4 status. Of the identified genes, 246 have not been reported previously as genes that lie downstream of TGF-beta. Genes that are involved in cell proliferation, adhesion, and motility were found to be regulated differentially with respect to S4KD and control cells. Cell migration induced by TGF-beta was inhibited in the S4KD cells, which might be associated with a different regulation of integrin beta7. The knock down of a specific gene using stable RNAi appears to be a promising tool for analysing endogenous gene function.
Collection C2: curated gene sets
      CGP: chemical and genetic perturbations
Source publication Pubmed 15592526   Authors: Jazag A,Ijichi H,Kanai F,Imamura T,Guleng B,Ohta M,Imamura J,Tanaka Y,Tateishi K,Ikenoue T,Kawakami T,Arakawa Y,Miyagishi M,Taira K,Kawabe T,Omata M
Exact source Table 3aS: Fold change >= 2
Related gene sets (show 3 additional gene sets from the source publication)

(show 1 gene sets from the same authors)
External links  
Organism Homo sapiens
Contributed by Leona Saunders (Broad Institute)
Source platform SEQ_ACCESSION
Dataset references  
Download gene set format: grp | text | gmt | gmx | xml
Compute overlaps (show collections to investigate for overlap with this gene set)
Compendia expression profiles Human tissue compendium (Novartis)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 108 genes
Gene families Categorize these 108 genes by gene family
Show members (show 108 members mapped to 108 genes)
Version history 3.0: First introduced

See MSigDB license terms here. Please note that certain gene sets have special access terms.