Human Gene Set: KANNAN_TP53_TARGETS_DN


Standard name KANNAN_TP53_TARGETS_DN
Systematic name M11403
Brief description Primary down-regulated targets of TP53 [GeneID=7157] in the H1299 (lung cancer) cell line.
Full description or abstract The transcriptional program regulated by the tumor suppressor p53 was analysed using oligonucleotide microarrays. A human lung cancer cell line that expresses the temperature sensitive murine p53 was utilized to quantitate mRNA levels of various genes at different time points after shifting the temperature to 32 degrees C. Inhibition of protein synthesis by cycloheximide (CHX) was used to distinguish between primary and secondary target genes regulated by p53. In the absence of CHX, 259 and 125 genes were up or down-regulated respectively; only 38 and 24 of these genes were up and down-regulated by p53 also in the presence of CHX and are considered primary targets in this cell line. Cluster analysis of these data using the super paramagnetic clustering (SPC) algorithm demonstrate that the primary genes can be distinguished as a single cluster among a large pool of p53 regulated genes. This procedure identified additional genes that co-cluster with the primary targets and can also be classified as such genes. In addition to cell cycle (e.g. p21, TGF-beta, Cyclin E) and apoptosis (e.g. Fas, Bak, IAP) related genes, the primary targets of p53 include genes involved in many aspects of cell function, including cell adhesion (e.g. Thymosin, Smoothelin), signaling (e.g. H-Ras, Diacylglycerol kinase), transcription (e.g. ATF3, LISCH7), neuronal growth (e.g. Ninjurin, NSCL2) and DNA repair (e.g. BTG2, DDB2). The results suggest that p53 activates concerted opposing signals and exerts its effect through a diverse network of transcriptional changes that collectively alter the cell phenotype in response to stress.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 11402317   Authors: Kannan K,Amariglio N,Rechavi G,Jakob-Hirsch J,Kela I,Kaminski N,Getz G,Domany E,Givol D
Exact source Table 2
Related gene sets (show 1 additional gene sets from the source publication)

(show 97 gene sets from the same authors)
External links
Filtered by similarity ?
Source species Homo sapiens
Contributed by Pablo Tamayo (MSigDB Team)
Source platform or
identifier namespace
AFFY_HuGene
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)

Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 22 genes
Gene families ? Categorize these 22 genes by gene family
Show members (show 24 source identifiers mapped to 22 genes)
Version history 3.0: Renamed from KANNAN_P53_DN

See MSigDB license terms here. Please note that certain gene sets have special access terms.