Gene Set: MACAEVA_PBMC_RESPONSE_TO_IR

Standard name MACAEVA_PBMC_RESPONSE_TO_IR
Systematic name M52
Brief description Genes up-regulated in human peripheral blood mononuclear cells (PBMC) at 8 h after exposure to 0.1 and 1.0 Gy dose of ionizing radiation.
Full description or abstract Peripheral blood mononuclear cells (PBMC) were isolated using Histopaque-1077 (Sigma-Aldrich) from 10 healthy donors (5 males, 5 females, aged 23-50). Isolated cells were resuspended at a density of 106 cells/ml and allowed to equilibrate to culture conditions at 37C in a humidified 5% CO2 atmosphere. PBMCs were then exposed to 0 (sham), 0.1 or 1.0 Gy of X-rays (250 keV - 1 mA, 1 mm Cu) at a dose rate of 0.26 Gy/min. After irradiation, cells were incubated at 37C in a humidified 5% CO2 atmosphere for 8 h before RNA extraction. Two weeks later, the experiment was repeated using fresh PBMCs from the same donors, resulting in a total of 60 RNA samples. Total RNA (250 ng) was used for hybridization to GeneChip Human Gene 1.0 ST arrays (Affymetrix). This gene set contains genes that were up-regulated after radiation exposure (3-way ANOVA, FDR <0.05).
Collection C2: curated gene sets
      CGP: chemical and genetic perturbations
Source publication Pubmed 26763932   Authors: Macaeva E,Saeys Y,Tabury K,Janssen A,Michaux A,Benotmane MA,Vos De WH,Baatout S,Quintens R
Exact source Table S1
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Organism Homo sapiens
Contributed by Roel Quintens (Belgian Nuclear Research Centre)
Source platform HuGene-1_0_st
Dataset references (show 1 datasets)
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Compendia expression profiles Human tissue compendium (Novartis)
NCI-60 cell lines (National Cancer Institute)
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Version history 6.2: changed members

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