The PRISM method is designed to enable high-throughput phenotypic analysis of cell lines. With the stable introduction of nucleotide barcodes into particular cell lines, phenotypic assays may be performed with pools of these cell lines and enumeration of barcodes reflects relative cell numbers at the end of the assay. This modular method allows interrogation of either particular cell types or comprehensive cell collections and may be performed in smaller laboratories or institutional screening platforms. Cell viability readout with PRISM is comparable to more traditional measurement methods performed in individual (unpooled) cell lines. PRISM has also been used to study drug response in xenograft models. Overall, PRISM makes practicable the deep or comprehensive profiling of cell responses across many different cell models and may facilitate analysis of genome-scale data to allow the elucidation of drug mechanisms and the discovery of predictive biomarkers.
Contact: Channing Yu, Todd Golub

Publication Info: 
High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines