Known Issues

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GSEA version 2

GSEA and Snow Leopard

Problem: GSEA version 2.0.4 does not run under Snow Leopard.

Solution: upgrade to GSEA v.2.0.6 or higher.

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Java heap space / OutOfMemoryError

Problem: When running an analysis in GSEA. the following error occurs:

---- Stack Trace ----

1. of exceptions: 1
   

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Java heap space------
java.lang.OutOfMemoryError: Java heap space

Cause: The error is either due to improper memory allocation, or because you have reached the limits on your machine.

Solutions:

1. Start GSEA by clicking the Launch button on the Downloads page of the GSEA web site.
2. Run GSEA on a more powerful computer.
3. Use no more than 1,000 permutations.
4. First, collapse gene identifiers to symbols using Chip2Chip tool, then run GSEA on the collapsed data set.
   When running GSEA on the collapsed dataset, make sure that 'Collapse dataset(s)' = false
5. First, create rank ordered list of genes outside GSEA, then run GSEA on the ranked list using GSEAPreranked tool
6. Use the -Xmx option to specify sufficient maximum amount of memory for the program by running GSEA from the command line.

 

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Firewall / FTP connection issues

Problem: When you try to access the CHIP annotation files or the Gene Set Database / MSigDB Browser, you see an error to the effect:

"Error listing Broad website//  Connection reset//"

Cause: This is probably because you are behind a network firewall or someother network configuration that prevents you from accessing FTP servers on port 500. The Broad chip files and gene sets are placed on a publically accessible Broad FTP server. The GSEA Java Desktop program tries to access the Broad FTP site to provide you easy access to the files but the network configuration blocks access.

Solutions:

1. Go to GSEA program main page > Options > Preferences and make sure that 'Connect over the internet' is checked.
2. See if you can temporarily disable your firewall when using GSEA.
3. Consult with your local network administrator to see if they have any suggestions or prior experience such issues.
4. Download the gene set (GMT) and CHIP files to your local file system as follows:
   1. Download this large zip file to your local file system. It contains all current (as of April 8, 2008) CHIP files. Expand it with a program like WinZip or gzip.
   2. Download this gene sets file to your local file system. It contains all current (MSigDB release 2.5) gene sets.
   3. Start GSEA.
   4. In GSEA, turn off the internet connection mode. Click Options>Preferences.

      On the General preferences page, clear the 'Connect over the Internet' option and click OK.

   5. Use the Load Data page to load the local annotation files and gen set files.
   6. On the Run GSEA page, select the local annotation files and gene set files rather than using the files from the GSEA website.

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"No probe called" error

Problem: When you run GSEA, sometimes the following errors appear in the log file:

ERROR - No Probe called: USP9X /// USP9Y on this chip (chip name is >GENE_SYMBOL<)
ERROR - Turning off subsequent error notifications

Solution: You can ignore these errors. The three slashes (///) indicate that the chip file contains ambiguous mappings, typical for Affymetrix notation, where a probe set on the chip cannot be mapped to exactly one HUGO gene symbol. GSEA displays this error and ignores such ambigous probes.

Avoid collapsing ranked list of features to gene symbols

Collapsing of ranked list is appropriate only if all its features have a one to one correspondence to human gene symbols. If more than one feature in the ranked list correspond to the same gene symbol, then the collapsing will change the original ranking order. Collapsing to gene symbols should be done BEFORE the ranked list of genes is made.

To ensure that the original ranking remains unchanged, we recommend making the ranked list with human gene symbols as gene identifiers and running GSEAPreranked with the parameter "Collapse dataset to gene symbols" set to "false".

 

Browse MSigDB doesn't load custom database XML files

Problem:You have created your own custom database XML file but it does not load into the GSEA browser.

GSEA version 1

java.lang.OutOfMemoryError (GSEA v1)

Problem: On the Mac, you can run GSEA from the command line, but when you attempt to use the GSEA application from the desktop you receive errors similar to the following:

---- Full Error Message ----
na

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Stack Trace ----

1. of exceptions: 1
   

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null------

java.lang.OutOfMemoryError

Solution: Corrected in GSEA v2. In GSEA v1, this is a memory issue with the gsea installer on the Mac. As a workaround, use the following command to launch the GSEA application rather than double clicking the icon:

java -Xmx1800m xapps.gsea.Main

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java.lang.NullPointerException (GSEA v1)

Problem: By default, a gene set enrichment analysis uses phenotype permutations. If you have too few samples for phenotype permutation, the following error occurs:

---- Stack Trace ----

1. of exceptions: 1
   

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null------

java.lang.NullPointerException
    at edu.mit.broad.genome.alg.DatasetStatsCore.calc2ClassCategoricalMetricMarkerScores(DatasetStatsCore.java:236)
    at edu.mit.broad.genome.alg.markers.PermutationTestBuilder.<init>(PermutationTestBuilder.java:94)
    at edu.mit.broad.genome.alg.gsea.KSTests.shuffleTemplate_canned_templates(KSTests.java:360)
    at edu.mit.broad.genome.alg.gsea.KSTests.shuffleTemplate(KSTests.java:291)
    at edu.mit.broad.genome.alg.gsea.KSTests.executeGsea(KSTests.java:156)
    at edu.mit.broad.genome.alg.gsea.KSTests.executeGsea(KSTests.java:130)
    at xtools.gsea.AbstractGsea2Tool.execute_one(AbstractGsea2Tool.java:103)
    at xtools.gsea.AbstractGsea2Tool.execute_one_with_reporting(AbstractGsea2Tool.java:137)
    at xtools.gsea.Gsea.execute(Gsea.java:111)
    at edu.mit.broad.xbench.tui.TaskManager$ToolRunnable.run(TaskManager.java:468)
    at java.lang.Thread.run(Unknown Source)

Solution: Corrected in GSEA v2. In GSEA v1, use gene_set permutation rather than phenotype permutation. For more information, see the description of the Permutation type parameter on the Run GSEA Page in the GSEA User Guide.

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GSEA-R

Error in memory.size

Problem: When running the example programs provided for R, the following error occurs:

[1] " *** Running GSEA Analysis..."
Error in memory.size(size) : don't be silly!: your machine has a 4Gb address limit

Cause: This is produced by the following line early in the GSEA.1.R file:

memory.limit(6000000000)

This line set the memory limit to a large size as a work around to a platform problem with an earlier R version.

Solution: The easiest fix is just to comment out that line:

# memory.limit(6000000000)

This will allocate the default amount of memory. If after this change the program runs out of memory, change the line to:

memory.limit(max. size in Mbytes available)

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16 warnings on R version 2.5 or higher

Problem: When running the example programs provided for R, the following warnings occur:

1: '\%' is an unrecognized escape in a character string
2: unrecognized escape removed from "Tag \%"
3: '\%' is an unrecognized escape in a character string
4: unrecognized escape removed from "Gene \%"
5: '\%' is an unrecognized escape in a character string
6: unrecognized escape removed from "\%"
7: '\%' is an unrecognized escape in a character string
8: unrecognized escape removed from " \%)"
9: '\.' is an unrecognized escape in a character string
10: '\.' is an unrecognized escape in a character string
11: unrecognized escapes removed from "\.report\."
12: '\.' is an unrecognized escape in a character string
13: '\.' is an unrecognized escape in a character string
14: unrecognized escapes removed from "\.report\."
15: '\.' is an unrecognized escape in a character string
16: unrecognized escape removed from "\."

Solution: You can ignore these warning messages. They occur when you have R version 2.5 and higher installed.

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GSEA on Linux

Browser links do not work under Linux

Problem: When running the GSEA desktop application under Linux, buttons and links that would normally open a browser window do not open the browser window.

Work-around: After running an analysis, you cannot click on the Success link to display the result. However, you can go to the directory that contains the analysis report output and open the index.html file in that directory.