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− | <h1><a name="_Toc127331830">Header and Footer</a></h1>
| + | [http://www.broadinstitute.org/gsea/ GSEA Home] | |
− | <p class="MsoNormal">The headers and footers would normally come over to html, but just for completeness, those formats would be</p> | + | [http://www.broadinstitute.org/gsea/downloads.jsp Downloads] | |
− | <ul>
| + | [http://www.broadinstitute.org/gsea/msigdb/ Molecular Signatures Database] | |
− | <li><!--[if !supportLists]--><span style="font-family: Symbol;"><span style="" /></span>Header: <span class="HeaderChar">header text</span></li>
| + | [http://software.broadinstitute.org/cancer/software/gsea/wiki/index.php/Main_Page Documentation] | |
− | <li><!--[if !supportLists]--><span class="FooterChar"><span style="font-family: Symbol;"><span style=""><span style="font-family: "Times New Roman"; font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"></span></span></span></span><!--[endif]-->Footer: <span class="FooterChar">footer text<o:p></o:p></span></li>
| + | [http://www.broadinstitute.org/gsea/contact.jsp Contact] |
| + | <br> |
| + | <p> Use the navigation bar on the left to display documentation on GSEA software, MSigDB database or GSEA/MSigDB web site. If you have comments or questions not answered by the [[FAQ]] or the [http://www.broadinstitute.org/gsea/doc/GSEAUserGuideFrame.html User Guide], contact us at [https://groups.google.com/group/gsea-help groups.google.com/group/gsea-help].</p> |
| + | |
| + | <ul> When contacting our team with questions about java GSEA programs, please send the following information: |
| + | <li> your computer's operation system |
| + | <li> version of java which you used to run GSEA |
| + | <li> detailed log transcript from the GSEA session in question |
| + | <p> to view the log, click [+] at the bottom of main screen of GSEA java desktop application, copy the text to a separate file and attach it to your request </p> |
| </ul> | | </ul> |
− | <h1><a name="_Toc127331831">Tables and Graphics</a></h1> | + | |
− | <p class="MsoNormal">Tables need to come over cleanly. It’s hard to capture all formats, but here’s a basic table:</p> | + | <h2>Where to start</h2> |
− | <table cellspacing="0" cellpadding="0" border="1" style="border: medium none ; border-collapse: collapse;" class="MsoTableGrid">
| + | <p> If you are new to GSEA, see the [http://www.broadinstitute.org/gsea/doc/desktop_tutorial.jsp Tutorial] for a brief overview of the software. |
− | <tbody>
| + | If you have a question, see the [[FAQ]] or the [http://www.broadinstitute.org/gsea/doc/GSEAUserGuideFrame.html User Guide]. The User Guide describes how to prepare data files, load data files, run the gene set enrichment analysis, and interpret the results. It also includes instructions for running GSEA from the command line and a Quick Reference section, which describes each window of the GSEA desktop application. <br /> |
− | <tr style="">
| + | </p> |
− | <td width="319" valign="top" style="border: 1pt solid windowtext; padding: 0in 5.4pt; width: 239.4pt;">
| + | <h3>Getting started with RNA-seq and GSEA</h3> |
− | <p class="MsoNormal"><strong style="">Column Header<o:p></o:p></strong></p>
| + | The GSEA method was originally developed for analysis of microarray data. In order to best adapt this method for RNA-sequencing data sets the GSEA team has developed a [[Using_RNA-seq_Datasets_with_GSEA|collection of guidelines and suggestions which describe how to properly handle these data.]] |
− | </td>
| + | <h2>MSigDB gene sets</h2> |
− | <td width="319" valign="top" style="border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; border-width: 1pt 1pt 1pt medium; padding: 0in 5.4pt; width: 239.4pt;">
| + | <p> Current release of the Molecular Signatures Database is divided into two parts, the MSigDB Human Collections, and the MSigDB Mouse collections. Release notes for the current version of the Human collections are available here: ([[MSigDB_v2023.1.Hs_Release_Notes|MSigDB v2023.1.Hs]]) and the release notes for the current version of the Mouse collections are available here: ([[MSigDB_v2023.1.Mm_Release_Notes|MSigDB v2023.1.Mm]]). For information about MSigDB and the gene sets, see the [http://www.broadinstitute.org/gsea/msigdb MSigDB web site]. </p> |
− | <p class="MsoNormal"><strong style="">Column Header<o:p></o:p></strong></p>
| + | <p> Please note that gene sets can change or become deprecated in subsequent releases of MSigDB. It is thus important to indicate the version of MSigDB to fully reference gene sets used in your study. </p> |
− | </td>
| + | |
− | </tr>
| + | <h2>Software</h2> |
− | <tr style="">
| + | <p>We provide the following software implementations of the GSEA method: |
− | <td width="319" valign="top" style="border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; border-width: medium 1pt 1pt; padding: 0in 5.4pt; width: 239.4pt;">
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− | <p class="MsoNormal">Blah, blah, blah.</p>
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− | <ul>
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− | <li><!--[if !supportLists]--><span style="font-family: Symbol;"><span style=""><span style="font-family: "Times New Roman"; font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"></span></span></span><!--[endif]-->Bulleted stuff, stuff, stuff.</li>
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− | <li><!--[if !supportLists]--><span style="font-family: Symbol;"><span style=""><span style="font-family: "Times New Roman"; font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"></span></span></span><!--[endif]-->Stuff, stuff, stuff.</li>
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− | </ul>
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− | </td>
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− | <td width="319" valign="top" style="border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 239.4pt;">
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− | <p class="MsoNormal">Blah, blah, blah.</p>
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− | <p style="" class="MsoListNumber"><!--[if !supportLists]--><span style="">1.<span style="font-family: "Times New Roman"; font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> </span></span><!--[endif]-->Numbered stuff, stuff, stuff.</p>
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− | <p class="MsoListNumber"><!--[if !supportLists]--><span style="">2.<span style="font-family: "Times New Roman"; font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"> </span></span><!--[endif]-->Stuff, stuff, stuff.</p>
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− | </td>
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− | </tr>
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− | <td width="319" valign="top" style="border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; border-width: medium 1pt 1pt; padding: 0in 5.4pt; width: 239.4pt;">
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− | <p class="MsoNormal">Blah, blah, blah.</p>
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− | </td>
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− | <td width="319" valign="top" style="border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 239.4pt;">
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− | <p class="MsoNormal">Blah, blah, blah.</p>
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− | </td>
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− | </tbody>
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− | </table>
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− | <p class="MsoNormal"><o:p> </o:p></p>
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− | <p class="MsoNormal">In Word, graphics are linked in files. They also need to come over:</p>
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− | <p class="MsoNormal"><!--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600"
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− | o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f"
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− | stroked="f">
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− | <v:f eqn="if lineDrawn pixelLineWidth 0" />
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− | <v:f eqn="sum @0 1 0" />
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− | <v:f eqn="sum 0 0 @1" />
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− | <v:f eqn="prod @2 1 2" />
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− | <v:f eqn="prod @3 21600 pixelWidth" />
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− | <v:f eqn="prod @3 21600 pixelHeight" />
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− | <v:f eqn="sum @0 0 1" />
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− | <v:f eqn="prod @6 1 2" />
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− | <v:f eqn="prod @7 21600 pixelWidth" />
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− | <v:f eqn="sum @8 21600 0" />
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− | <v:f eqn="prod @7 21600 pixelHeight" />
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− | <v:f eqn="sum @10 21600 0" />
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− | </v:formulas>
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− | <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect" />
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− | <o:lock v:ext="edit" aspectratio="t" />
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− | </v:shapetype><v:shape id="_x0000_i1025" type="#_x0000_t75" style='width:14.25pt;
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− | height:14.25pt'>
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− | <v:imagedata src="file:///C:\DOCUME~1\Aravind\LOCALS~1\Temp\msohtml1\01\clip_image001.gif"
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− | o:href="images/icon-file-res.gif" />
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− | </v:shape><![endif]--><!--[if !vml]--><img width="19" height="19" v:shapes="_x0000_i1025" src="file:///C:/DOCUME~1/Aravind/LOCALS~1/Temp/msohtml1/01/clip_image001.gif" alt="" /><!--[endif]--></p>
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− | <p class="MsoNormal">In rare cases, graphics appear in tables. Here’s an example:</p> | |
− | <table cellspacing="0" cellpadding="0" border="1" style="border: medium none ; margin-left: 5.4pt; border-collapse: collapse;" class="MsoTableGrid">
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− | <tbody>
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− | <td width="72" valign="top" style="border: 1pt solid windowtext; padding: 0in 5.4pt; width: 0.75in;">
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− | <p class="MsoNormal"><!--[if gte vml 1]><v:shape id="_x0000_i1026" type="#_x0000_t75"
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− | style='width:14.25pt;height:14.25pt'>
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− | <v:imagedata src="file:///C:\DOCUME~1\Aravind\LOCALS~1\Temp\msohtml1\01\clip_image001.gif"
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− | o:href="images/icon-file-res.gif" />
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− | </v:shape><![endif]--><!--[if !vml]--><img width="19" height="19" v:shapes="_x0000_i1026" src="file:///C:/DOCUME~1/Aravind/LOCALS~1/Temp/msohtml1/01/clip_image001.gif" alt="" /><!--[endif]--></p>
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− | <td width="559" valign="top" style="border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; border-width: 1pt 1pt 1pt medium; padding: 0in 5.4pt; width: 419.4pt;">
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− | <p class="MsoNormal">Expression dataset (res) </p>
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− | </td>
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− | <td width="72" valign="top" style="border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; border-width: medium 1pt 1pt; padding: 0in 5.4pt; width: 0.75in;">
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− | <p class="MsoNormal"><!--[if gte vml 1]><v:shape id="_x0000_i1027" type="#_x0000_t75"
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− | style='width:14.25pt;height:14.25pt'>
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− | <v:imagedata src="file:///C:\DOCUME~1\Aravind\LOCALS~1\Temp\msohtml1\01\clip_image002.gif"
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− | o:href="images/icon-file-gct.gif" />
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− | </v:shape><![endif]--><!--[if !vml]--><img width="19" height="19" v:shapes="_x0000_i1027" src="file:///C:/DOCUME~1/Aravind/LOCALS~1/Temp/msohtml1/01/clip_image002.gif" alt="" /><!--[endif]--></p>
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− | </td>
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− | <td width="559" valign="top" style="border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 419.4pt;">
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− | <p class="MsoNormal">Expression dataset (gct)</p>
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− | </td>
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− | <p class="MsoNormal"><!--[if gte vml 1]><v:shape id="_x0000_i1028" type="#_x0000_t75"
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− | o:href="images/icon-file-pcl.gif" />
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− | </v:shape><![endif]--><!--[if !vml]--><img width="18" height="18" v:shapes="_x0000_i1028" src="file:///C:/DOCUME~1/Aravind/LOCALS~1/Temp/msohtml1/01/clip_image003.gif" alt="" /><!--[endif]--></p>
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− | </td>
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− | <td width="559" valign="top" style="border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; border-width: medium 1pt 1pt medium; padding: 0in 5.4pt; width: 419.4pt;">
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− | <p class="MsoNormal">Expression dataset (pcl)</p>
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− | </td>
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− | </tr>
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− | </tbody>
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− | </table>
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− | <h1><a name="_Toc127331832">Content</a></h1>
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− | <p class="MsoNormal">The introduction to each section that has subsections, should provide hyperlinks to the subsections. </p> | |
− | <p class="MsoNormal">This section describes how to work with project directories in GenePattern:</p>
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| <ul> | | <ul> |
− | <li><!--[if !supportLists]--><span style="font-family: Symbol;"><span style=""><span style="font-family: "Times New Roman"; font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"></span></span></span><!--[endif]--><a href="#_Opening_a_Project_Directory">Projects Pane</a></li> | + | <li>Java desktop application -- Easy-to-use graphical interface that can be run from the [http://www.broadinstitute.org/gsea/downloads.jsp Downloads] page. The [http://www.broadinstitute.org/gsea/doc/GSEAUserGuideFrame.html User Guide] fully describes this application in detail. |
− | <li><!--[if !supportLists]--><span style="font-family: Symbol;"><span style=""><span style="font-family: "Times New Roman"; font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-size-adjust: none; font-stretch: normal;"></span></span></span><!--[endif]--><a href="#_Opening_a_Project_Directory_1">Opening a Project Directory</a></li> | + | </li> |
| + | <li>Java jar file -- Command line interface that can be downloaded from the [http://www.broadinstitute.org/gsea/downloads.jsp Downloads] page. See [http://software.broadinstitute.org/gsea/doc/GSEAUserGuideTEXT.htm#_Running_GSEA_from Running GSEA from the Command Line] in the <i>User Guide</i> for details. This might be useful for analyzing several datasets sequentially, analyzing large datasets, or running analyses on a compute cluster.</li> |
| + | <li>R-GSEA -- R implementation of GSEA that can be downloaded from the [http://www.broadinstitute.org/gsea/downloads_archive.jsp Archived Downloads] page. This implementation is intended for experienced computational biologists who may want to explore the underlying algorithm. The [[R-GSEA_Readme|R-GSEA Readme]] provides brief instructions and support is limited. Please note that this implementation is not actively maintained or supported.</li> |
| </ul> | | </ul> |
| + | </p> |
| + | <p>Thank you for your interest in GSEA,<br> |
| + | The GSEA Team</p> |
GSEA Home |
Downloads |
Molecular Signatures Database |
Documentation |
Contact
Use the navigation bar on the left to display documentation on GSEA software, MSigDB database or GSEA/MSigDB web site. If you have comments or questions not answered by the FAQ or the User Guide, contact us at groups.google.com/group/gsea-help.
Where to start
If you are new to GSEA, see the Tutorial for a brief overview of the software.
If you have a question, see the FAQ or the User Guide. The User Guide describes how to prepare data files, load data files, run the gene set enrichment analysis, and interpret the results. It also includes instructions for running GSEA from the command line and a Quick Reference section, which describes each window of the GSEA desktop application.
Getting started with RNA-seq and GSEA
The GSEA method was originally developed for analysis of microarray data. In order to best adapt this method for RNA-sequencing data sets the GSEA team has developed a collection of guidelines and suggestions which describe how to properly handle these data.
MSigDB gene sets
Current release of the Molecular Signatures Database is divided into two parts, the MSigDB Human Collections, and the MSigDB Mouse collections. Release notes for the current version of the Human collections are available here: (MSigDB v2023.1.Hs) and the release notes for the current version of the Mouse collections are available here: (MSigDB v2023.1.Mm). For information about MSigDB and the gene sets, see the MSigDB web site.
Please note that gene sets can change or become deprecated in subsequent releases of MSigDB. It is thus important to indicate the version of MSigDB to fully reference gene sets used in your study.
Software
We provide the following software implementations of the GSEA method:
- Java desktop application -- Easy-to-use graphical interface that can be run from the Downloads page. The User Guide fully describes this application in detail.
- Java jar file -- Command line interface that can be downloaded from the Downloads page. See Running GSEA from the Command Line in the User Guide for details. This might be useful for analyzing several datasets sequentially, analyzing large datasets, or running analyses on a compute cluster.
- R-GSEA -- R implementation of GSEA that can be downloaded from the Archived Downloads page. This implementation is intended for experienced computational biologists who may want to explore the underlying algorithm. The R-GSEA Readme provides brief instructions and support is limited. Please note that this implementation is not actively maintained or supported.
Thank you for your interest in GSEA,
The GSEA Team