V2 notes from user testing

Notes from testing/user feedback of beta v2 software

Fill in gotchas, observations of user behavior etc for the v2 software

(dont enter bugs here, use Gsea_enhancements instead


I ran gsea v2 and the results not identical to PNAS published results. Theres a bug!

Probabaly not

1) your results modestly different - FDRS change a bit or the order of top gene sets is a little different
This is because the FDR for a small number of sets can be a bit choppy in the tail. So, a small diff (few percent) in FDR is not unusual as the random seed used from run to run is different
Additionally, the mapping databases have been updated to reflect the latest releases of NetAffx, Unigene and related chip annotations. These changes might have affected the mapping of gene sets from native ids to gene symbols and also the collapsing of datasets from probe ids to gene symbols. Our internal tests show that these effects are minor but nevertheless could account for small differences in FDR

2) The results are hugely different
Something is wrong and its most likely your fault.


Whats the relation b/w GSEA and the new stuff (MSigDB Browser, Chip2Chip)

msigdb, chip2chip etc are not needed for vanilla gsea. You can run gsea with any gene sets.
They help find gene sets and map them b/w platforms.

For gsea, simply the 3 steps. No need to do mapping etc. Even collapsing is now built in.


GSEA Java Needs

Needs 1.4+
Preferred to use 1.5

So if you launch on a mac and see the error message unsupported class version it means that your mac (or windows) doesnt not have the correct java version.
For the mac: http://docs.info.apple.com/article.html?artnum=302412