This guide teaches you how to launch an analysis in FireCloud with your own workflows and data. If you do not have your own workflows or data, you can take advantage of the Featured workspaces to see how pipelines are configured and run a dummy analysis on a small sample file. We have the GATK4 Best Practice pipelines and our $5 genome analysis pipeline featured. Learn how to use Featured Workspaces in this video.
Note: Before you can run the steps here or in the video, you must:
Each step is followed by a screenshot of the action. Some steps are linked to tutorials where you can read more detail.
|Method Repository||Create New Method|
Now you will edit the workspace to prepare it for your analysis. Upload your data (BAMs, vcfs, etc.) to the Workspace’s Google bucket (video or written instructions). A link to your bucket is in the workspace's Summary tab.
Update your workspace's data model to display links to the files you uploaded in Step 3. Create Tab-separated value files (saved as .txt) that organize your metadata by participant, sample, pair, sets of participants, samples, or pairs.
See the attached templates for the import format and required fields for each entity. Once your metadata files are setup, navigate to the Data tab in the workspace, click "Import Metadata", and import the participant file (required), sample file (optional), pair file(optional), participant_set file (optional), other set files, etc. in that order.
With the data and metadata setup, you are ready to configure the method in the workspace. Import the method you created in Step 1.
|Import Method||Use Blank Configuration|
Configure the method using the WDL inputs json to see the attribute values that you need to fill in. Notice the format for each attribute type in the screenshot below. See this written or this video tutorial for a full list of formatting requirements and to learn how to reference files in the data model and/or workspace attributes in your analysis.
Monitor the job. Refresh the page to see submission, workflow, and task status updates. If the submission status turns red, check the workflow's failures section to see a description of what occurred. The log files are available per workflow and are very helpful for debugging. Read more about workflow statuses and troubleshooting here.
Done?! If there are no errors, the workflow(s) will complete and the workspace status will be green. You can take a look at the outputs in the Monitor tab within each workflow or in the Data tab (if the method configuration is set up to write there).
Try these steps out and let us know about your experience. If you find something missing or different than you expected, please let us know in the Comments section.