Allele-specific strand bias estimated using Fisher's exact test (AS_FS)
Strand bias is a type of sequencing bias in which one DNA strand is favored over the other, which can result in incorrect evaluation of the amount of evidence observed for one allele vs. the other.
The AS_FisherStrand annotation is one of several methods that aims to evaluate whether there is strand bias in the data. It uses Fisher's Exact Test to determine if there is strand bias between forward and reverse strands for the reference or alternate allele, and does so separately for each alternate allele.
The output is a Phred-scaled p-value. The higher the output value, the more likely there is to be bias. More bias is indicative of false positive calls.
See the method document on statistical tests for a more detailed explanation of this application of Fisher's Exact Test.
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